Web6-Diazo-5-oxo-L-nor-Leucine （L-6-Diazo-5-oxonorleucine; DON） 是一种谷氨酰胺拮抗剂，能不可逆地抑制谷氨酰胺的分解作用。 6-Diazo-5-oxo-L-nor-Leucine 具有较好的抗癌活性 （尤其是胰腺癌），能降低肿瘤细胞的自我更新潜力和转移能力。
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WebPharmacological inhibition of GFAT decreased renal cortex O -GlcNAcylation, proteinuria, and albuminuria in SHR. Using a proximal tubule cell line, we observed that increased O -GlcNAcylation reduced megalin surface expression and albumin endocytosis in vitro, and the effects were correlated in vivo. WebJan 25, 2024 · Decreasing O-GlcNAcylation by inhibitors of the glutamine:fructose-6-phosphate aminotransferase (GFAT), blocks cardiomyocyte hypertrophy, mimicking AMPK activation. Conversely,...
WebThe GFAT inhibitors DON and azaserine (40 μ m) as well as an inhibitor of direct protein glycosylation, BG (1 m m), significantly decreased SMC proliferation induced by 30 m m glucose (44.2% inhibition with DON, 20.1% with azaserine, 66.3% with BG) , as measured on the fourth day after stimulation of HASMC with high glucose. WebMar 7, 2024 · Using a label-free phosphoproteomic analysis, we identified glutamine:fructose-6-phosphate amidotransferase 1 (GFAT1) as an AMPK substrate. …
WebIn addition to GFAT 1, emerging evidence shows that GFAT 2 is upregulated in hearts subject to ISO stress. Furthermore, concomitant infusion of ISO with 5-diazo-oxo-norleucine (DON; GFAT... WebDec 12, 2024 · Knockdown of GFAT2 suppresses ISO-induced cardiomyocyte hypertrophy, accompanied by suppression of Akt O-GlcNAcylation and activation. Knockdown of GFAT2 does not affect anti-hypertrophic effect by Akt inhibition. Administration of glucosamine, a substrate of HBP, induces protein O-GlcNAcylation, Akt activation, and cardiomyocyte …
WebOct 1, 2024 · DON is a GFAT inhibitor and acts upstream in HBP. To confirm that the observed effects are indeed due to HBP inhibition and not an artifactual outcome of DON …
WebJul 26, 2024 · Suppression of O -GlcNAcylation by the glutamine fructose-6-phosphate amidotransferase (GFAT) inhibitor, 6‐diazo‐5‐oxo‐ l ‐norleucine (DON), or OGT inhibitor, OSMI-1, impaired memory function, and inhibited FC-induced PKA/CREB activation. how fine to grind coffee for pour overWebSuppressing GFAT activity resulted in downregulation of BiP that activated inositol-requiring enzyme 1α, a sensor protein of UPR, and exacerbated cisplatin-induced cell … how fine should coffee be for drip makerWebDec 17, 2024 · Diazo-5-oxo-L-norleucine (DON: 50 μM, 12 h), an inhibitor of GFAT, also suppressed protein O-GlcNAcylation and Akt phosphorylation in cardiomyocytes treated with ISO or PE ( Figures S6 A and S6B). Transient activation of hypertrophic signaling is … how fine to grind weedWebAlloxan inhibits Gfat1-induced hypertrophic growth a Inhibition of O-GlcNAcylation with alloxan led to a decrease in cardiomyocyte growth from Gfat1 overexpression. Adenovirus-mediated ... higher rate pension tax relief claimWebSuppression of O-GlcNAcylation by the glutamine fructose-6-phosphate amidotransferase (GFAT) inhibitor, 6-diazo-5-oxo-L-norleucine (DON), or OGT inhibitor, OSMI-1, impaired memory function, and inhibited FC-induced PKA/CREB activation. DON additionally reduced the amplitude of baseline field excitatory postsynaptic potential (fEPSP) and ... how finfet reduces short channel effectsWebDiazo-5-oxo-L-norleucine (DON) is an inhibitor of GFAT, and treatment with DON significantly downregulated both basal and 10058/10074-induced increases in protein glycosylation (Figure 1C, E, F, and H). Interestingly, … how finfet worksWebOct 15, 2009 · Both GFAT protein and activity, measured by the glutamate dehydrogenase method , increased during glucose deprivation (Fig. 5 A). Treatment of cells with the GFAT inhibitor DON completely blocked O-GlcNAcylation under glucose deprivation (Fig. 5 B). how fingerprint recognition works